Title : Antibacterial and antibiofilm activity of Turpentine nanoemulsion against methicillin resistant Staphylococcus aureus
Turpentine essential oil (TEO) is a commercially available product having various application due to its ethno-botanical and ethnopharmacological properties. In the present study, we performed chemical composition of TEO by Gas Chromatography-Mass Spectrometry (GC-MS). Further, TEO was nanoemulsified, encapsulated and characterized by droplet size, PDI, Zeta potential and transmittance. The obtained turpentine nanoemulsion (TNE) was investigated for its antibacterial and antibiofilm potentiality against methicillin-resistant Staphylococcus aureus (MRSA), a model biofilm-forming microorganism. Small micellar TEO nanoparticles were succesfully formed with a mean droplet size ranging from 22.52 to 26.54nm. Thermodynamic stability studies revealed homogeneous dispersion of the droplets size confirming the stability of TNEs. The developed nano-emulsions displayed two fold enhanced antagonistic activity against S. aureus in comparison with TEOs, with minimum inhibitory concentration (MIC) values at 0.039% (v/v) against MRSA. Additionally, TNEs displayed potent antibiofilm activity against MRSA strains with percent biofilm disruption of around 70.83%. Findings from this study validates the phytomedicinal significance of turpentine nanoemulsions and envisage its exploration as a natural and cost-effective strategy against bacterial biofilms in medical and industrial sectors.
What will audience learn from your presentation?
- Preparation of Nanoemulsion from Turpentine essential oil (TEO)
- Characterization of Turpentine Nanoemulsion (TNE)
- Evaluation of antibacterial efficacy of TNE (compared to the TEO) against methicillin-resistant Staphylococcus aureus
- Assessment of antibiofilm potential of TNE
- TNE can be used as an effective agent to prevent surface contaminations with bacterial biofilm in various sectors (valorization of TEO as a disinfectant).