International Conference and Expo on

Clinical Microbiology

June 17-18, 2022 | Online Event

ICCM 2022

Antibacterial and antibiofilm activity of Turpentine nanoemulsion against methicillin resistant Staphylococcus aureus

Speaker at Clinical Microbiology 2022 - Abderrahmen Merghni
University of Tunis El Manar, Tunisia
Title : Antibacterial and antibiofilm activity of Turpentine nanoemulsion against methicillin resistant Staphylococcus aureus


Turpentine essential oil (TEO) is a commercially available product having various application due to its ethno-botanical and ethnopharmacological properties. In the present study, we performed chemical composition of TEO by Gas Chromatography-Mass Spectrometry (GC-MS). Further, TEO was nanoemulsified, encapsulated and characterized by droplet size, PDI, Zeta potential and transmittance. The obtained turpentine nanoemulsion (TNE) was investigated for its antibacterial and antibiofilm potentiality against methicillin-resistant Staphylococcus aureus (MRSA), a model biofilm-forming microorganism. Small micellar TEO nanoparticles were succesfully formed with a mean droplet size ranging from 22.52 to 26.54nm. Thermodynamic stability studies revealed homogeneous dispersion of the droplets size confirming the stability of TNEs. The developed nano-emulsions displayed two fold enhanced antagonistic activity against S. aureus in comparison with TEOs, with minimum inhibitory concentration (MIC) values at 0.039% (v/v) against MRSA. Additionally, TNEs displayed potent antibiofilm activity against MRSA strains with percent biofilm disruption of around 70.83%. Findings from this study validates the phytomedicinal significance of turpentine nanoemulsions and envisage its exploration as a natural and cost-effective strategy against bacterial biofilms in medical and industrial sectors.

What will audience learn from your presentation?

  • Preparation of Nanoemulsion from Turpentine essential oil (TEO)
  • Characterization of Turpentine Nanoemulsion (TNE) 
  • Evaluation of antibacterial efficacy of TNE (compared to the TEO) against methicillin-resistant Staphylococcus aureus
  • Assessment of antibiofilm potential of TNE 
  • TNE can be used as an effective agent to prevent surface contaminations with bacterial biofilm in various sectors (valorization of TEO as a disinfectant).



Dr. Abderrahmen studied Biotechnology at the Monastir University, Tunisia and graduated as MS in 2009. He then joined the research group of Prof. Mastouri at the Faculty of Pharmacy of Monastir, at the Monastir University. He received his PhD degree in 2016 at the same institution. After one year he obtained the position of an Associate Professor of Microbiology at the Faculu of Medicine of Tunis, Tunisia. He has published more than 23 research articles in SCI(E) journals.