Title : Ultraluminescent Biophotonics assays for In Flow Escherichia Coli bacteria detection and counting towards perspectives on Single Biostructure analysis at molecular level
In this presentation it is showed Research and Development related with the design and synthesis of Ultraluminescent Nano-emitters based on Metal Enhanced Fluorescence (MEF) phenomena. It is showed gold Core-shell Nanoparticles with silica spacers and modified Fluorescent Silica shells with Enhanced and stable Luminescent properties. These Nano-emitters were used as Nanolabellers for Escherichia Coli bacteria. In this manner it was achieved Bright and Ultraluminescent Nano-Biostructures that afforded to faster detection within low bacteria concentrations at the level of single bacterium detection by Laser Fluorescence Microscopy. In this context it is highlighted and discussed the importance of the Optical properties of Nano-labellers and Biostructures for targeted applications from the Nano-Biostructure formation. Thus, it is showed In Flow Cytometry detection of Nano-Biostructures at varied levels of concentrations. In addition, it is discussed perspectives about how it could be managed these Hybrid Nano-Biostructures to record additional information from single Biostructure analysis. In this manner it is leaded to new potential approaches within Biophotonics by Targeted Light Delivery through Biostructures that just not improve only Biodetection, but as well it increases the power of analysis towards Single Cells applications.
What will audience learn from your presentation?
- In the presentation the audience could incorporate proofs of concepts and new approaches from the control of the Nanoscale toward developments within Nanophotonics and Biophotonics.
- In this manner, the design and synthesis of functional Nanoparticles could lead to Nano-Biolabelling with improved imaging resolution.
Ultraluminescence generation based on Metal Enhanced Fluorescence (MEF) is discussed for faster detection and counting of Escherichia Coli bacteria.