Title : WGS based analysis of bovine mastitis-related MRSA in Thuringia, Germany
Abstract:
In animal husbandry, acute and chronic staphylococcal mastitis cause massive financial losses for the producer due to the reduced milk yield of the infected cow, price reductions due to reduced milk quality up to a ban on milk sales and significantly increased premature loss of animals. A multi-parametric staphylococcal rapid test is to be developed to match this one-health problem. The probability that a dairy cow will be culled increases significantly after three udder treatments. A major cause of these infections is Staphylococcus (S.) aureus. In the case of sanitation, the quarter milk samples from 10% of the animals in a herd (assumed infection rate of approx. 25%) with cell counts greater than 250,000 cells/mL milk must be examined, as well as dry cows, heifers and newcomers.
The aim of the study was to compare the genotypes, antimicrobial resistance profiles, and virulence factors of MRSA strains from clinical and subclinical bovine mastitis in dairy farms in Thuringia, Germany. Whole-genome sequencing (WGS) of strains was conducted and the sequence data were analyzed regarding antimicrobial resistance and virulence associated genes to draw conclusions for a current situation of bovine clinical and subclinical mastitis infections in dairy herds in the state and the potential public health risk. Furthermore, mapping the possible phylogenetic relations between MRSA strains from various farms as well as within one farm.
What will audience learn from your presentation?
- Audience will expand their knowledge about current situation of bovine mastitis in Germany.
- Audience will get a knowledge about the important resistance genes in bovine mastitis associated pathogens.
- provide new information to assist in a study-design to solve mastitis problem in dairy herds.
