Title : Antimicrobial activity of Zinc oxide nano particles against multi drug resistant Escherichia coli clinical isolates
Multi drug resistance (MDR) is becoming a global problem for public health which threatens the lives of hospitalized individuals as well as increases health care costs and long-time treatment. MDR in Gram-negative bacteria, chiefly Enterobacteriaceae such as E. coli, has become one of the major global concerns. Contrary to the synthetic drugs, some inorganic nanoparticles (NPs) are not associated with many side effects and have a great therapeutic potential to treatment of infectious caused by resistant microbes
We aimed to study Zinc oxide nano particles (ZnO NPs) as an alternative antibacterial agent for controlling MDR E. coli.
The study was carried out on 40 MDR E. coli isolates collected from patients admitted to Mansoura University Hospitals.
The selected isolates were tested for the effect of different concentrations of ZnO NPs alone as an antibacterial agent and the combined effect of antibiotics and ZnO NPs as an antibacterial agent by the agar well diffusion method. Effect of ZnO NPs on E. coli isolates were confirmed by Transmission Electron Microscope.
Of all used concentrations of ZnO NPs, the concentration (4.176 g/l) was the most active one with inhibition zones diameter ranged between (14-26 mm) and the second concentration (1.392 g/l) caused inhibition zones diameter ranged between (14-24mm), followed by (0.464, 0.154, 0.051, 0.017, 0.0057 g/l) respectively. The antibacterial activity of antibiotics such as meropenem, piperacillin tazobactam, cefipime, amoxicillin clavulanic acid, ciprofloxacin, gentamycin, amikacin, azteronam, nitrofurantoin, sulphamethoxazole trimethoprim, cefaclor, tetracyclin and norfloxacin respectively against E. coli was increased considerably in the presence of ZnO NPs while the antibacterial activity of cefotaxime was not increased considerably in the presence of ZnO NPs.
ZnO NPs were effective in controlling MDR E. coli isolates in vitro. Increasing the concentrations of ZnO NPs lead to more inhibition of E. coli growth. Further studies are recommended to study its efficacy in vivo.
Audience Take Away:
- Explore antibacterial activity of Zinc oxide nano particles (ZnO NPs) on MDR E. coli isolates .
- Clarify the combined effect of ZnO NPs and different antibiotics on MDR E. coli isolates.
- Explore morphological changes on E. coli treated with ZnO NPs by transmission EM.