Microorganisms live in biofilms that adhere to biotic and abiotic surfaces in most natural, clinical, and industrial settings. Biofilms are formed when microorganisms attach to surfaces. Biofilm is a complex microbiome structure that adheres to the surface and contains different bacterial colonies or single types of cells in a group. One of the implications of bacterial biofilm communities, which contribute to chronic diseases, is bacterial antibiotic resistance. Biofilms are clusters of microbial cells that are attached to a surface in a virtually irreversible manner, i.e., they cannot be removed by gently lifting the surface. Biofilms can form on living or non-living surfaces and can be found in a variety of environments, including natural, industrial, and hospital settings. Planktonic cells of the same organism, on the other hand, are single cells that float or swim in a liquid media and are physiologically separate from microbial cells developing in a biofilm. Biofilms can build as dental plaque on the teeth of most mammals, causing tooth decay and gum disease.